Fig 2: Chaperone binding correlates to SMP binding. SMP binding correlates to binding of TF (A, Pearson's r = 0.94), HSP70 (B, Pearson's r = 0.92), or HSP90 (C, Pearson's r = 0.97) on a panel of antibodies isolated from internal screening campaigns against hen egg lysozyme.

Fig 3: Chaperone binding correlates to SMP binding in approved and preclinical candidates. SMP binding was correlates to binding of TF (A, Pearson's r = 0.81), HSP70 (B, Pearson's r = 0.72), HSP90 (C, Pearson's r = 0.88), or SCP (D, Pearson's r = 0.94) on a subset of a panel of approved and preclinical antibodies. Red points are used to highlight particular clones with interesting binding properties.

Fig 4: Chaperone proteins predict mouse clearance rates. Median fluorescence intensity (MFI) of trigger factor (A, Spearman's ρ = 0.43), HSP70 (B, Spearman's ρ = 0.60), HSP90 (C, Spearman's ρ = 0.65), or SMP (D, Spearman's ρ = 0.72) binding for each antibody correlates with mouse clearance rates from a previous study comparing SMP binding to mouse pharmacokinetics.6